The Netherlands international midfielder sustained the injury during Barcelona's training session ahead of their La Liga clash with Villarreal. Initial tests suggest a muscle tear that could keep him out for four weeks.
De Jong's absence is a significant blow to Hansi Flick's squad, as it comes just when the manager had nearly a fully fit team available. The captain would miss crucial matches including the Copa del Rey semi-final second leg against Atletico Madrid and potential Champions League ties.